The purpose of this research is to find an optimal number and configuration of readers in RFID based 3D Indoor Positioning System. The system applies a Hybrid Scene Analysis - Neural Network algorithm to estimate target's position with a desired accuracy. The system's accuracy and cost depend on a number of utilized readers and their arrangement. Readers' deployment is crucial for the localization accuracy too. The system optimization enhances the system cost-efficiency. The arrangement analysis was based on simulations and validated by physical experiment. The results of this research define a trade-off between a number of readers and their deployment and the system performance in terms of localization accuracy.
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